An <scp>auxin‐mediated</scp> regulatory framework for <scp>wound‐induced</scp> adventitious root formation in tomato shoot explants

Adventitious roots (ARs) are produced from non-root tissues in response to different environmental signals, such as abiotic stresses, or after wounding, a complex developmental process that requires hormonal crosstalk. Here, we characterized AR formation young seedlings of Solanum lycopersicum cv. ‘Micro-Tom’ whole root excision by means physiological, genetic and molecular approaches. We found regulated basipetal auxin transport the shoot local biosynthesis triggered wounding both required for re-establishment internal gradients within vasculature. This promotes cell proliferation at distal cambium near wound well-defined positions basal hypocotyl during narrow window. In addition, pre-established pattern differential responses along apical-basal axis an yet unknown cell-autonomous inhibitory pathway contribute temporal spatial patterning newly formed ARs on isolated explants. Our work provides experimental outline dissection wound-induced tomato, species is suitable identification gene regulatory networks via forward reverse genetics